Catalog Number: 20-2015-xx
Description: Ac-dC-CPG 1000
5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 1000
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Not Applicable
FREQUENTLY ASKED TECHNICAL QUESTION
QUESTION: What is the procedure for deprotecting with AMA?
RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium
hydroxide and aqueous MethylAmine. With AMA the cleavage of the
oligonucleotide from the support is accomplished in 5 minutes at room
temperature. The deprotection step is carried out at 65°C for a
further 5 minutes. Deprotection can also be carried out at lower
temperatures as shown in Table 1. In all cases, no base modification
has been observed.
TABLE 1: DEPROTECTION WITH AMA
|10 min ||55°C|
|90 min ||25°C|
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