Glen Research Product Information

Catalog Number: 20-2013-xx
Description: Ac-dC-CPG 500

5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 500

 
 
F.W.: 289.18 

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.

Deprotection: Deprotect using the protocol required by the nucleobases.

Storage: Controlled room temperature or lower, dry

Stability in Solution: Not Applicable

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


20-2113-42
4x0.2µm
40.00
20-2113-41
4x1.0µm
60.00
20-2113-13
1x10µm
100.00
20-2013-01
0.1g
9.00
20-2013-02
0.25g
20.00
20-2013-10
1.0g
75.00
20-2213-42
4x0.2µm
40.00
20-2213-41
4x1.0µm
60.00
20-2213-14
1x15µm
150.00

Related Document(s)

  

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What is the procedure for deprotecting with AMA?

RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium

hydroxide and aqueous MethylAmine. With AMA the cleavage of the

oligonucleotide from the support is accomplished in 5 minutes at room

temperature. The deprotection step is carried out at 65°C for a

further 5 minutes. Deprotection can also be carried out at lower

temperatures as shown in Table 1. In all cases, no base modification

has been observed.

TABLE 1: DEPROTECTION WITH AMA

TimeTemperature
5 min65°C
10 min 55°C
30 min37°C
90 min 25°C


 

 

VIEW PRINTABLE VERSION

 

 


  

01/21/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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