Glen Research Product Information

Catalog Number: 11-1330-xx
Description: Cis-syn Thymine Dimer Phosphoramidite

5'-Dimethoxytrityl-2'-deoxy-5,6-dihydro-Thymidine-(cis-syn-cyclobutanyl)- 5'->3'-(methyl-phosphoryl)-2'-deoxy-5,6-dihydro-Thymidine-3'-[methyl- (N,N-diisopropyl)]-phosphoramidite

Formula: C49H63N5O15P2 
M.W.: 1024.01 
F.W.: 608.39 

Diluent: Anhydrous Acetonitrile

Coupling: 10 minute coupling recommended.

Deprotection: 1. Remove the methyl phosphate groups by treating the support with thiophenol/triethylamine/THF (1:2:2) v/v/v for 45 minutes at room temperature. Wash the support with THF 1X, methanol 5X, acetonitrile 3X and dry with air or argon. 2. Cleave and deprotect the oligo in the dark with ammonium hydroxide at room temperature under conditions appropriate to remove the protecting groups on the nucleobases.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


11-1330-95
50µm
2100.00
11-1330-90
100µm
4200.00
11-1330-02
0.25g
10200.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
11-1330-020.25grams.25grams2.446840.825.518.5513.63.4
11-1330-90100µmoles.102grams120127.55.4541
11-1330-9550µmoles.051grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
11-1330-020.25grams.25grams3.64.0766.441.530.184.15
11-1330-90100µmoles.102grams1.5.0723.614.7510.731.48
11-1330-9550µmoles.051grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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