1. Remove the methyl phosphate groups by treating the support with thiophenol/triethylamine/THF (1:2:2) v/v/v for 45 minutes at room temperature. Wash the support with THF 1X, methanol 5X, acetonitrile 3X and dry with air or argon.
2. Cleave and deprotect the oligo in the dark with ammonium hydroxide at room temperature under conditions appropriate to remove the protecting groups on the nucleobases.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.