Glen Research Product Information

Catalog Number: 10-5961-xx
Description: Methylene Blue II Phosphoramidite

3-Butyl-3-[2-(2-cyanoethyl)(N,N-diisopropyl)phosphoramidityl)ethyl]amino-7-(2-(4,4'-dimethoxytrityloxy)ethyl)-7-(butylamino)phenothiazin-5-ium chloride

Formula: C54H70ClN5O5PS 
M.W.: 967.67 
F.W.: 489.57 

Diluent: Anhydrous Acetonitrile

Coupling: 3 minute coupling time recommended. The use of UltraMILD monomers are required. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). Some branching off the Methylene Blue will occur when incorporated internally within an oligo, so we recommend the dye be placed at or near the 5' terminus of the oligo sequence.

Deprotection: UltraMILD deprotection: 0.05M potassium carbonate in methanol (60-4600-30) for 4 hours at room temperature

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 Days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-5961-95
50µm
310.00
10-5961-90
100µm
595.00
10-5961-02
0.25g
1500.00

Related Document(s)

  

Literature Highlight(s)

 

 

VIEW PRINTABLE VERSION

 

 


  

01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2019