Coupling: 3 minute coupling time recommended. The use of UltraMILD monomers are required. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). Some branching off the Methylene Blue will occur when incorporated internally within an oligo, so we recommend the dye be placed at or near the 5' terminus of the oligo sequence.
Deprotection: UltraMILD deprotection: 0.05M potassium carbonate in methanol (60-4600-30) for 4 hours at room temperature