Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Complete the deprotection using the protocol required by the nucleobases. Note, the use of methylamine in ultrafast deprotection protocols will result in degradation to the dye and is not recommended.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: 'Black Hole Quencher', 'BHQ-0', 'BHQ-1', 'BHQ-2' and 'BHQ-3' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.