Glen Research Product Information

Catalog Number: 10-5931-xx
Description: 5'-BHQ-1 Phosphoramidite

4'-(2-Nitro-4-toluyldiazo)-2'-methoxy-5'-methyl-azobenzene-4''-(N-ethyl)-N-ethyl- 2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite

Formula: C34H45N8O5
M.W.: 676.75 
F.W.: 538.49 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

Please Note: 'Black Hole Quencher', 'BHQ-0', 'BHQ-1', 'BHQ-2' and 'BHQ-3' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-5931-95
50µm
100.00
10-5931-90
100µm
200.00
10-5931-02
0.25g
700.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the physical properties of the various black hole quenchers?

RESPONSE:

Quencher lmax (nm)E260 (L/mol.cm)Emax (L/mol.cm)
BHQ-0 4937,70034,000
BHQ-15348,00034,000
BHQ-2 5798,00038,000
BHQ-367213,000 42,700

REFERENCE(S):
Glen Report 17.1


QUESTION: Dye Quencher Plot

RESPONSE:Dye Quencher Plot


DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-5931-020.25grams.25grams3.69109.6765.841.1329.9121.935.48
10-5931-90100µmoles.068grams120127.55.4541
10-5931-9550µmoles.034grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-5931-020.25grams.25grams5.51.07103.864.8847.186.49
10-5931-90100µmoles.068grams1.5.0723.614.7510.731.48
10-5931-9550µmoles.034grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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