Glen Research Product Information

Catalog Number: 10-4960-xx
Description: 3-Cyanovinylcarbazole Phosphoramidite (CNVK)

5'-O-(4,4'-Dimethoxytrityl)-1'-(3-cyanovinylcarbazol-9-yl)-2'-deoxy-β-D-ribofuranosyl- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C50H53N4O6
M.W.: 836.95 
F.W.: 396.33 

Diluent: Anhydrous Acetonitrile

Coupling: Regular. The use of UltraMILD monomers are preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: If UltraMILD reagents were used, deprotect in 0.05M Potassium Carbonate in Methanol for 4 hours at Room Temperature OR for 2 hours at Room Temperature in 30% Ammonium Hydroxide. If standard bases were used, deprotection in Ammonium Hydroxide at Room Temperature for 24-36 hours will give acceptable yields. For photo cross-linking conditions please see http://www.glenresearch.com/GlenReports/GR23-14.html.

Storage: Freezer storage, -10 to -30°C, dry in the dark. Light sensitive material.

Stability in Solution: 1-2days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-4960-95
50µm
200.00
10-4960-90
100µm
390.00
10-4960-02
0.25g
1125.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-4960-020.25grams.25grams2.9986.3351.832.3823.5517.274.32
10-4960-90100µmoles.084grams120127.55.4541
10-4960-9550µmoles.042grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-4960-020.25grams.25grams4.46.0782.851.7537.645.18
10-4960-90100µmoles.084grams1.5.0723.614.7510.731.48
10-4960-9550µmoles.042grams.75.078.65.383.91.54
Mermade
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity30nm200nm1000nm
Approximate Number of Additions
10-4960-020.25grams.25grams4.46.0784.452.7538.36
10-4960-90100µmoles.084grams1.5.0725.215.7511.45
10-4960-9550µmoles.042grams.75.0710.26.384.64

 

 

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01/18/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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