Glen Research Product Information

Catalog Number: 10-3501-xx
Description: 1-Me-A-CE Phosphoramidite

5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-Adenosine, 2'-O-TBDMS- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C48H63ClN7O7PSi 
M.W.: 944.57 
F.W.: 344.24 

Diluent: Anhydrous Acetonitrile

Coupling: 15 minute coupling time recommended. Monomers that allow for UltraMILD deprotection are recommended. (Pac-A:10-3000-xx, Ac-C: 10-3015-xx, iPr-Pac-G: 10-3021-xx, U: 10-3030-xx ).

Deprotection: 2 M Ammonia in methanol, 24 hours at room temperature using UltraMILD phosphoramidites and UltraMILD Cap A is recommended. Alternatively, 60 hours at room temperature using standard phosphoramidites and Cap A will give acceptable results. To remove the 2’-TBDMS group, dry down the supernatant, treat with 1.5 mL of 1 M TBAF in THF for 18 hours at room temperature, quench with the addition of 1 mL of 1.5 M ammonium acetate and desalt (2x) using a Glen Gel-Pak™ 2.5 Desalting Column or equivalent.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-3501-95
50µm
190.00
10-3501-90
100µm
380.00
10-3501-02
0.25g
975.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-3501-9550µmoles.047grams.53.3321.25.91.67.17
10-3501-90100µmoles.094grams120127.55.4541
10-3501-020.25grams.25grams2.65754528.1320.45153.75
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-3501-9550µmoles.047grams.75.078.65.383.91.54
10-3501-90100µmoles.094grams1.5.0723.614.7510.731.48
10-3501-020.25grams.25grams3.95.0772.645.38334.54

 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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