Glen Research Product Information

Catalog Number: 10-3420-xx
Description: 2'-F-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-deoxyGuanosine, 2'-fluoro-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite

Formula: C44H53FN7O8
M.W.: 857.91 
F.W.: 347.19 

Diluent: Anhydrous Acetonitrile

Coupling: 3 minute coupling time recommended.

Deprotection: Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature. (Note - heating in AMA will lead to some degradation of the 2'-Fluoro nucleotides.)

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

Please Note: A license may be required from Ionis Pharmaceuticals, Inc. to incorporate 2'-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 6,005,087; 6,531,584 and foreign equivalents.

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-3420-02
0.25g
100.00
10-3420-05
0.5g
200.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-3420-020.25grams.25grams2.9183.6750.231.3822.8216.734.18
10-3420-050.5grams.5grams5.83181108.667.8849.3636.29.05
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-3420-020.25grams.25grams4.35.0780.650.3836.645.04
10-3420-050.5grams.5grams8.7.07167.6104.7576.1810.48

 

 

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01/18/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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