Glen Research Product Information

Catalog Number: 10-3072-xx
Description: 6-Thio-G-CE Phosphoramidite

5'-Dimethoxytrityl-N2-isobutyryl-6-(2-cyanoethyl)thio-Guanosine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C53H71N8O8PSSi 
M.W.: 1039.31 
F.W.: 361.26 

Diluent: Anhydrous Acetonitrile

Coupling: 12 minute coupling time recommended. Use 0.02M Iodine to avoid degradation and Ac-C (10-3015).

Deprotection: Treat column with 1M DBU in ACN for 4 hrs at RT, rinse and dry support. Then deprotect with 50mM NaSH in AMA at 65°C for 10 minutes. Complete using RNA deprotection. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-3072-95
50µm
250.00
10-3072-90
100µm
500.00
10-3072-02
0.25g
1200.00

Related Document(s)


Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-3072-020.25grams.25grams2.416740.225.1318.2713.43.35
10-3072-90100µmoles.104grams120127.55.4541
10-3072-9550µmoles.052grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-3072-020.25grams.25grams3.59.07N/AN/A13.63N/A
10-3072-90100µmoles.104grams1.5.07N/AN/A4.92N/A
10-3072-9550µmoles.052grams.75.07N/AN/A1.79N/A

 

 

VIEW PRINTABLE VERSION

 

 


  

01/18/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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