Coupling: 12 minute coupling time recommended. See Technical Bulletin
Deprotection: RNA deprotaction using Ammonium Hydroxide:MethylAmine (AMA) 1:1 at 65°C for 10 minutes. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.