Glen Research Product Information

Catalog Number: 10-3011-xx
Description: Zebularine-CE Phosphoramidite

5'-Dimethoxytrityl-pyrimidin-2-one-riboside, 2'-O-TBDMS- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C45H61N4O8PSi 
M.W.: 845.05 
F.W.: 290.17 

Diluent: Anhydrous Acetonitrile

Coupling: 12 minute coupling time is recommended. Monomers that allow for UltraMild deprotection must be used. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: Cleave and remove base protection in 30% Ammonium Hydroxide:EtOH (3:1) for 4 hours at Room Temperature. Complete 2' deprotection per RNA Technical Bulletin. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-3011-95
50µm
125.00
10-3011-90
100µm
250.00
10-3011-02
0.25g
650.00

Related Document(s)


Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-3011-9550µmoles.042grams.53.3321.25.91.67.17
10-3011-90100µmoles.085grams120127.55.4541
10-3011-020.25grams.25grams2.9685.3351.23223.2717.074.27
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-3011-9550µmoles.042grams.75.07N/AN/A1.79N/A
10-3011-90100µmoles.085grams1.5.07N/AN/A4.92N/A
10-3011-020.25grams.25grams4.42.07N/AN/A17.08N/A

 

 

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01/20/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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