Coupling: 12 minute coupling time is recommended. Monomers that allow for UltraMild deprotection must be used. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: Cleave and remove base protection in 30% Ammonium Hydroxide:EtOH (3:1) for 4 hours at Room Temperature. Complete 2' deprotection per RNA Technical Bulletin.
See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.