Glen Research Product Information

Catalog Number: 10-1994-xx
Description: 6-Fluorescein Serinol Phosphoramidite

3-Dimethoxytrityloxy-2-(3-(6-carboxy-(di-O-pivaloyl-fluorescein)propanamido)propyl)- 1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Formula: C67H75N4O14
M.W.: 1191.3 
F.W.: 582.45 

Diluent: Anhydrous Acetonitrile

Coupling: 12-15 minute coupling time recommended.

Deprotection: Use ammonium hydroxide and deprotect as required by nucleobases. When using AMA, a small amount of a non-fluorescent impurity will be formed. To eliminate this impurity, first deprotect with ammonium hydroxide for 30 minutes at room temperature, add an equal volume of 40% methylamine and then complete the deprotection as required by the nucleobases - e.g. 10 minutes at 65°C or 2 hours at room temperature for standard bases.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

Please Note: This product is covered by US Patent No.: 8,394,948.

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1994-95
50µm
165.00
10-1994-90
100µm
295.00
10-1994-02
0.25g
595.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1994-020.25grams.25grams2.156.673421.2515.4511.332.83
10-1994-90100µmoles.119grams120127.55.4541
10-1994-9550µmoles.06grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1994-020.25grams.25grams3.13.0756.235.1325.553.51
10-1994-90100µmoles.119grams1.5.0723.614.7510.731.48
10-1994-9550µmoles.06grams.75.078.65.383.91.54

 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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