Glen Research Product Information

Catalog Number: 10-1983-xx
Description: Psoralen C6 Phosphoramidite

6-[4'-(Hydroxymethyl)-4,5',8-trimethylpsoralen]-hexyl-1-O-(2-cyanoethyl)- (N,N-diisopropyl)-phosphoramidite

Formula: C30H43N2O6
M.W.: 558.65 
F.W.: 420.40 

Diluent: Anhydrous Acetonitrile

Coupling: 10 minute coupling time recommended.

Deprotection: Mild: 0.4M Methanolic NaOH 17hr @ RT or ammonium hydroxide 24h @ RT Note: NaOH is not compatible with dmf protecting groups.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1983-90
100µm
195.00
10-1983-02
0.25g
495.00

Related Document(s)


FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What is the sequence requirement for psoralen mediated cross-linking in double stranded DNA?

RESPONSE:Psoralens are a class of naturally occurring heterocyclic compounds which intercalates between bases in double-stranded or triple stranded DNA. Upon exposure to long wavelength light (350 nm) Psoralen forms covalent linkages to Thymidine (cyclobutane linkage). Psoralen is a bifunctional reagent and can form either a monoadduct, linking one adjacent thymidine on the same or complimentary strand, or a diadduct, linking adjacent thymidines on the same or complimentary strands. Diadducts formed between adjacent thymidines are photoreversable with short wavelength UV light (254 nm).

Type of Adduct

Optimal Sequence

Cross-linking

Reversing

Wavelength

Wavelength

Monoadduct

5’-Psoralen-XAX-XXX-

350 nm

NA

Diadduct

5’-Psoralen-TAX-XXX-

350 nm

254 nm

Note: For adducts between double-stranded DNA use Psoralen C210-1982.
For adducts between triple-stranded DNA use Psoralen C6 which has a longer linker arm 10-1983.

REFERENCE(S):
1. U. Pieles and U. Englisch, Nucleic Acids Res., 1989, 17, 285.


QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html


DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1983-020.25grams.25grams4.4813681.65137.0927.26.8
10-1983-90100µmoles.056grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1983-020.25grams.25grams6.68.07127.279.557.827.95
10-1983-90100µmoles.056grams1.5.0723.614.7510.731.48

 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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