Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes. Removal of the PDA requires 30 minutes at Room Temperature with AMA.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days
Please Note: PDA amino-modifiers were developed by Stefan Pitsch and ReseaChem GmbH (S. Berger), Patent pending.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.