Glen Research Product Information

Catalog Number: 10-1939-xx
Description: Spermine Phosphoramidite

N¹-[4-(4,4'-Dimethoxytrityloxy)butyl]-N¹,N4,N9,N¹²-tetrakis(trifluoroacetyl)- spermine-N¹²-butyl-4-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C56H73F12N6O9
M.W.: 1233.17 
F.W.: 408.52 

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.

Coupling: 3 minute coupling time recommended.

Deprotection: Prior to cleavage, treat support with 10% diethylamine in acetonitrile for 5 minutes at room temperature and rinse with acetonitrile to remove the cyanoethyl protecting groups. Then deprotect using 30% ammonium hydroxide as required by nucleobases. (Note a minimum of 4 hours at room temperature, or approximately 30 minutes at 55°C, is required to remove the TFA protecting groups.)

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Please Note: "Spermine phosphoramidite" synthon is the subject matter of U.S. Divisional Patent Application 14/745,871, European Patent No. EP 1973927 B1 and foreign equivalents for which Polyplus-transfection is the co-owner. Product is sold for research purposes only. Product shall not be used to manufacture oligospermine oligonucleotide conjugates for use in diagnostics, clinical or commercial applications including use in humans. There is no implied license to manufacture oligospermine oligonucleotide conjugates for diagnostic, clinical or commercial applications, including but not limited to contract research. Please contact Polyplus-transfection at licensing@polyplus-transfection.com to obtain a license for such use.

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1939-95
50µm
145.00
10-1939-90
100µm
270.00
10-1939-02
0.25g
525.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1939-9550µmoles.062grams.53.3321.25.91.67.17
10-1939-90100µmoles.123grams120127.55.4541
10-1939-020.25grams.25grams2.0354.3332.620.3814.8210.872.72
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1939-9550µmoles.062grams.75.078.65.383.91.54
10-1939-90100µmoles.123grams1.5.0723.614.7510.731.48
10-1939-020.25grams.25grams3.03.0754.233.8824.643.39

 

 

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01/18/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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