Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect as required by nucleobases.WARNING: As with other trityl-protected amines, drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_MMT_Amino_Modifiers.pdf).
Storage: Refrigerated storage, maximum of 2-8°C, dry
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.