Glen Research Product Information

Catalog Number: 10-1927-xx
Description: Abasic II Phosphoramidite

5-O-Dimethoxytrityl-1-O-tert-butyldimethylsilyl-2-deoxyribose- 3-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C41H59N2O7PSi 
M.W.: 750.98 
F.W.: 196.1 

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.

Coupling: 6 minute coupling time recommended.

Deprotection: Synthesize using acetyl-protected dC and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 min OR Synthesize using dmf-dG and deprotect in EtOH/30% Ammonium Hydroxide (1:3) for 24 hrs at room temperature. To generate the abasic site, dry the oligo down and take up in 0.25mL 80% Acetic acid. After 30 min at room temperature, add 0.25mL water and let sit for an additional 4 hrs. Quench with 0.5mL 2M TEAA and desalt on a Glen Gel-Pak™ column or equivalent. Store the oligo at pH 6-7 at 4°C or frozen. Do not dry; drying results in cleavage of the abasic site. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Abasic II-1.pdf).

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1927-95
50µm
80.00
10-1927-90
100µm
150.00
10-1927-02
0.25g
475.00

Related Document(s)


Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1927-9550µmoles.038grams.53.3321.25.91.67.17
10-1927-90100µmoles.075grams120127.55.4541
10-1927-020.25grams.25grams3.3397.6758.636.6326.6419.534.88
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1927-9550µmoles.038grams.75.078.65.383.91.54
10-1927-90100µmoles.075grams1.5.0723.614.7510.731.48
10-1927-020.25grams.25grams4.97.079358.1342.275.81

 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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