Diluent: Anhydrous Acetonitrile Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect as required by nucleobases.
WARNING: Drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.