Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: If the final DMT is removed during synthesis, deprotection under standard conditions will eliminate the Solid CPR II to yield a 5' Phosphate. If the DMT is retained, see Technical Bulletin for removal procedure (http://www.glenresearch.com/Technical/TB_CPR_II.pdf).
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: No data available
Please Note: Solid Chemical Phosphorylation Reagent II and related supports are covered by European Patent: EP0816368.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.