Glen Research Product Information

Catalog Number: 10-1900-xx
Description: Chemical Phosphorylation Reagent

2-[2-(4,4'-Dimethoxytrityloxy)ethylsulfonyl]ethyl-(2-cyanoethyl)- (N,N-diisopropyl)-phosphoramidite

Formula: C34H45N2O7PS 
M.W.: 656.77 
F.W.: 79.98 

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: As required by nucleobases. Complete deprotection to the 5’-phosphate requires a minimum of 17 hr @ RT or 4 hr @ 55 °C in ammonium hydroxide, 30 minutes at 65 °C in AMA (Ammonium hydroxide/40% methylamine 1:1 v/v), or 8 hr @ RT in K2CO3/MeOH. Note: the DMT group is removed during deprotection and is not available for reverse phase purification - e.g., GlenPak™ purification.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1900-90
100µm
50.00
10-1900-02
0.25g
160.00

Related Document(s)


Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1900-020.25grams.25grams3.81113.6768.242.633122.735.68
10-1900-90100µmoles.066grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1900-020.25grams.25grams5.68.07107.26748.736.7
10-1900-90100µmoles.066grams1.5.0723.614.7510.731.48

 

 

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12/12/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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