Glen Research Product Information

Catalog Number: 10-1564-xx
Description: 5-Formyl dC III CE Phosphoramidite

5'-O-(dimethoxytrityl)-N4-(4-methoxybenzoyl)-5-(1,3-dioxan-2-yl)- 2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite

Formula: C51H60N5O11
M.W.: 950.02 
F.W.: 317.19 FW: 375.27 (acetal) 

Diluent: Anhydrous Acetonitrile

Coupling: 3 minute coupling time is recommended.

Deprotection: In 30% ammonium hydroxide, use dmf-dG during synthesis and deprotect for 17 hours at room temperature. If deprotection in sodium hydroxide is required for use in oligos containing other epigenetic bases, use ibu-dG and Ac-dC during synthesis and deprotect using 0.4 M NaOH in MeOH/water 4:1 (v/v) for 17 hours at room temperature. Removal of acetal protecting group: if sodium hydroxide was used during deprotection, desalt the oligo and dry down, otherwise, dry down directly. Take up oligo in 80% acetic acid in water and let react for 6 hours at 20°C

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1564-95
50µm
360.00
10-1564-90
100µm
700.00
10-1564-02
0.25g
1800.00

Related Document(s)


Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1564-9550µmoles.048grams.53.3321.25.91.67.17
10-1564-90100µmoles.095grams120127.55.4541
10-1564-020.25grams.25grams2.6374.3344.627.8820.2714.873.72
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1564-9550µmoles.048grams.75.078.65.383.91.54
10-1564-90100µmoles.095grams1.5.0723.614.7510.731.48
10-1564-020.25grams.25grams3.93.0772.245.1332.824.51

 

 

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01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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