Deprotection: In 30% ammonium hydroxide, use dmf-dG during synthesis and deprotect for 17 hours at room temperature. If deprotection in sodium hydroxide is required for use in oligos containing other epigenetic bases, use ibu-dG and Ac-dC during synthesis and deprotect using 0.4 M NaOH in MeOH/water 4:1 (v/v) for 17 hours at room temperature. Removal of acetal protecting group: if sodium hydroxide was used during deprotection, desalt the oligo and dry down, otherwise, dry down directly. Take up oligo in 80% acetic acid in water and let react for 6 hours at 20°C
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.