Glen Research Product Information

Catalog Number: 10-1539-xx
Description: DBCO-dT-CE Phosphoramidite

5'-Dimethoxytrityl-5-[(6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)- capramido-N-hex-6-yl)-3-acrylimido]-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C69H80N7O11
M.W.: 1214.57 
F.W.: 773.77 

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)

Coupling: 12 minute coupling time recommended. Note the DBCO-dT is susceptible to damage by iodine during oxidation. To prevent loss of the DBCO label, use 0.5M CSO in anhydrous acetonitrile (40-4632-xx) and a 3 minute oxidation time for best results.

Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65°C or 17 hours at room temperature. AMA (ammonium hydroxide/40% methylamine 1:1) for 2 hours at room temperature will give acceptable results.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1539-95
50µm
250.00
10-1539-90
100µm
485.00
10-1539-02
0.25g
975.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1539-9550µmoles.061grams.53.3321.25.91.67.17
10-1539-90100µmoles.121grams120127.55.4541
10-1539-020.25grams.25grams2.0655.3333.220.7515.0911.072.77
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1539-9550µmoles.061grams.75.078.65.383.91.54
10-1539-90100µmoles.121grams1.5.0723.614.7510.731.48
10-1539-020.25grams.25grams3.07.075534.38253.44

 

 

VIEW PRINTABLE VERSION

 

 


  

01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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