Coupling: 12 minute coupling time recommended. Note the DBCO-dT is susceptible to damage by iodine during oxidation. To prevent loss of the DBCO label, use 0.5M CSO in anhydrous acetonitrile (40-4632-xx) and a 3 minute oxidation time for best results.
Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65°C or 17 hours at room temperature. AMA (ammonium hydroxide/40% methylamine 1:1) for 2 hours at room temperature will give acceptable results.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.