Deprotection: Synthesize using acetyl-protected dC (10-1015-xx). Prior to cleavage, treat support with 10% diethylamine in ACN for 5 minutes at room temperature and rinse with ACN to remove the cyanoethyl protecting groups. Then cleave and deprotect the oligo using 30% ammonium hydroxide/40% methylamine 1:1 (AMA) for 2 hours at room temperature. Prior to use, reduce any disulfide formation using 100 mM TCEP or DTT for 30 minutes at room temperature.
Storage: Refrigerated storage, maximum of 2-8°C, dry
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.