Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: To label amine on column, treat support successively with 1 ml each for 10 minutes, 10% diethylamine/ACN (to remove cyanoethyl protection) and 20% piperidine/DMF to remove Fmoc protection. Rinse support with DMF and ACN to remove piperidine. Dissolve NHS ester (5-10 fold excess) in 0.5 ml appropriate solvent containing 1% diisopropylethylamine and incubate for 2-4 hours @ 35 oC with CPG to label amine. Rinse support to remove excess label and cleave/deprotect as normal or with conditions compatible with coupled label.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.