Glen Research Product Information

Catalog Number: 10-1536-xx
Description: Fmoc Amino-Modifier C6 dT

5'-Dimethoxytrityl-5-[N-((9-fluorenylmethoxycarbonyl)-aminohexyl)-3-acrylimido]- 2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C63H73N6O11
M.W.: 1121.28 
F.W.: 458.41(NH2) 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: To label amine on column, treat support successively with 1 ml each for 10 minutes, 10% diethylamine/ACN (to remove cyanoethyl protection) and 20% piperidine/DMF to remove Fmoc protection. Rinse support with DMF and ACN to remove piperidine. Dissolve NHS ester (5-10 fold excess) in 0.5 ml appropriate solvent containing 1% diisopropylethylamine and incubate for 2-4 hours @ 35 oC with CPG to label amine. Rinse support to remove excess label and cleave/deprotect as normal or with conditions compatible with coupled label.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1536-90
100µm
180.00
10-1536-02
0.25g
360.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1536-90100µmoles.112grams120127.55.4541
10-1536-020.25grams.25grams2.236136.622.8816.6412.23.05
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1536-90100µmoles.112grams1.5.0723.614.7510.731.48
10-1536-020.25grams.25grams3.33.0760.237.6327.363.76

 

 

VIEW PRINTABLE VERSION

 

 


  

01/20/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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