Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: In 30% ammonium hydroxide, deprotect for 2 hours at 65 °C. In 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA), synthesize using acetyl-protected dC (10-1015-xx) and deprotect at 65 °C for 20 minutes.
Storage: Refrigerated storage, maximum of 2-8°C, dry and in the dark
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.