Glen Research Product Information

Catalog Number: 10-1504-xx
Description: def-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-diethylformamidine-2'-deoxyAdenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C45H57N8O6
M.W.: 836.97 
F.W.: 313.21 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection:  In 30% ammonium hydroxide, deprotect for 2 hours at 65 °C. In 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA), synthesize using acetyl-protected dC (10-1015-xx) and deprotect at 65 °C for 20 minutes.

Storage: Refrigerated storage, maximum of 2-8°C, dry and in the dark

Stability in Solution: 1-2 Days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1504-02
0.25g
15.00
10-1504-05
0.5g
30.00
10-1504-10
1.0g
60.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1504-020.25grams.25grams2.9986.3351.832.3823.5517.274.32
10-1504-050.5grams.5grams5.97185.67111.469.6350.6437.139.28
10-1504-101.0gram1grams11.95385231144.381057719.25
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1504-020.25grams.25grams4.46.0782.851.7537.645.18
10-1504-050.5grams.5grams8.92.07172107.578.1810.75
10-1504-101.0gram1grams17.83.07350.2218.88159.1821.89

 

 

VIEW PRINTABLE VERSION

 

 


  

01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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