Coupling: 10 minute coupling time. CleanAmp™ monomers must be used with UltraMild phosphoramidites and Cap Mix.
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Glen Report 21.2 for details. (http://www.glenresearch.com/GlenReports/GR21-2.pdf)
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours
Please Note: CleanAmp™ is a trademark of TriLink BioTechnologies, Inc. CleanAmp™ products or portions thereof are covered by TriLink pending Patent Applications, US 2007281308 and WO2007139723, US Provisional Patent Application Serial # 61/056,324 and US Patent 6762298 licensed from the Department of Health and Human Services. CleanAmp™ products are sold exclusively for R & D use by the purchaser. They may not be resold, distributed or re-packaged. No license is granted or implied with the purchase of this product. Amplification methods used in connection with Polymerase Chain Reaction (“PCR”) Process are covered by many patents. It may be necessary to obtain a separate license for certain patented applications in which the product is used. CleanAmp™ Licenses are available directly from TriLink BioTechnologies.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.