QUESTION: What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?
RESPONSE:1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.
2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)
3. Oxidation: Following synthesis
Step 1: React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes
Step 2: Remove solution from support
Step 3: React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes
Step 4: Flush column with acetonitrile and blow dry support
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.