Glen Research Product Information

Catalog Number: 10-1200-xx
Description: dA-H-Phosphonate, TEA Salt

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine, 3'-H-phosphonate, TEA salt

Formula: C44H51N6O8
M.W.: 822.9 
F.W.: 313.21 

Diluent: Acetonitrile/Pyridine (1:1)

Coupling: H-Phosphonate Synthesis Protocol

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: ~1week

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1200-02
0.25g
40.00
10-1200-05
0.5g
80.00

Related Document(s)


FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?

RESPONSE:1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.

2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)

3. Oxidation: Following synthesis

Step 1: React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes

Step 2: Remove solution from support

Step 3: React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes

Step 4: Flush column with acetonitrile and blow dry support


DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1200-020.25grams.25grams21686.67412257.5187.27137.3334.33
10-1200-050.5grams.5grams421386.67832520378.18277.3369.33
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1200-020.25grams.25grams5.0693.658.542.555.85
10-1200-050.5grams.5grams10.06193.61218812.1

 

 

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01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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