Coupling: 15 minute coupling time using 0.25M DCI (30-3150-xx) is recommended. Using the phosphoramidite-based capping reagent Unicap (10-4410-xx), cap using a regular coupling time and then oxidize with 0.5M CSO for 3 minutes for best results.
Deprotection: Pretreat synthesis column with 1.5% DBU in anhydrous acetonitrile for 60 minutes at room temperature. Rinse with acetonitrile and dry with argon. Deprotect using 40% methylamine for 2 hours at room temperature.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This product is covered by US Patents 6,693,187 and 7,067,641, patents pending and foreign counterparts owned by Lievre Cornu. Purchase of all or any of these products includes a limited license to use the product solely in the manufacture of oligonucleotides for RESEARCH USE ONLY and its use is not authorized nor intended for diagnostic or therapeutic use.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.