Coupling: 3 minute coupling time is recommended. Due to the steric hindrance at the 5' hydroxyl, the coupling time for the phosphoramidite immediately following the addition of the 8,5'-Cyclo-dA should be increased to 6 minutes.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.