Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) for 10 minutes at 65°C is recommended. (To prevent modification of dC by Methylamine, use Ac-dC (10-1015-xx) during synthesis.)
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours
Please Note: This product is covered by patents or patents pending owned by Ionis Pharmaceuticals, Inc. ("Ionis"). Purchase of this product includes a limited license to use this product solely as an intermediary in the manufacture of oligomers containing 6 or more nucleosides ("Oligonucleotides") for internal research. This license specifically excludes (and you have no right to use this product or Oligonucleotides for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product or Oligonucleotides), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreign counterpart), (c) resale (including sale of any products or services that incorporate this product or Oligonucleotides) or (d) gene functionalization activities (including products or services that incorporate data derived from gene functionalization activities) if such activities have commercial application, any and all of which require a separate license from Ionis. Neither this product nor any product created through its use may be used in human clinical trials.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.