Glen Research Product Information

Catalog Number: 10-1096-xx
Description: Thymidine Glycol CE Phosphoramidite

5'-Dimethoxytrityl-2'-deoxy-(5R,6S)-5,6-bis(t-butyldimethylsilyloxy)- 5,6-dihydroThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite

Formula: C52H79N4O10PSi2 
M.W.: 1007.36 
F.W.: 338.21 

Diluent: Anhydrous Acetonitrile

Coupling: 3 minute coupling time is recommended. Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: Cleave and deprotect the oligonucleotide in 30% Ammonium Hydroxide for 2 hours at Room Temperature. Dry down and dissolve the residue in 0.5 mL of TEA.3HF and keep at 40° C overnight to remove the TBDMS protecting groups. Add an equal volume of dH2O and desalt on a Glen Gel-Pak™ column or equivalent.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1096-95
50µm
180.00
10-1096-90
100µm
360.00
10-1096-02
0.25g
975.00

Related Document(s)

  

Literature Highlight(s)

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1096-020.25grams.25grams2.4869.3341.62618.9113.873.47
10-1096-90100µmoles.101grams120127.55.4541
10-1096-9550µmoles.05grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1096-020.25grams.25grams3.7.0767.642.2530.734.23
10-1096-90100µmoles.101grams1.5.0723.614.7510.731.48
10-1096-9550µmoles.05grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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