Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR deprotect in Ammonium Hydroxide for 2 hours at room temperature to remove the acetyl protecting group and complete the deprotection under temperatures and times suitable to remove the protecting groups of the nucleobases.
Storage: Refrigerated storage, maximum of 2-8°C, dry
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.