Glen Research Product Information

Catalog Number: 10-1070-xx
Description: O6-Me-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-O6-methyl-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C45H56N7O8
M.W.: 853.97 
F.W.: 343.24 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: Treat the CPG with 10% DBU in anhydrous methanol at Room Temperature for 5 days in the dark to cleave and deprotect the oligo. 1) Prepare a solution of 10% DBU in anhydrous methanol. DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene) is available from Aldrich (139009-25G) as is anhydrous methanol (322415-100ML). Note: 10% DBU in anhydrous methanol is not compatible with dmf-protecting groups. 2) Transfer the CPG to a clean, dry glass vial and add 1 mL of the 10% DBU solution. 3) Seal the vial and leave for 5 days in the dark at room temperature. 4) Transfer the contents to a micro centrifuge tube and reduce under vacuum to a small volume. 5) Add 1mL of 10mM aqueous sodium hydroxide solution and desalt or purify the oligonucleotide using standard procedures.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1070-90
100µm
105.00
10-1070-02
0.25g
255.00

Related Document(s)


DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1070-020.25grams.25grams2.9384.3350.631.632316.874.22
10-1070-90100µmoles.085grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1070-020.25grams.25grams4.37.078150.6336.825.06
10-1070-90100µmoles.085grams1.5.0723.614.7510.731.48

 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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