Glen Research Product Information

Catalog Number: 10-1057-xx
Description: TAMRA-dT

5'-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)- 3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C73H83N8O13
M.W.: 1311.48 
F.W.: 870.85 

Diluent: 10% THF in Anhydrous Acetonitrile

Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). Allow 15 minutes for the product to go into solution. A 6 minute coupling time is recommended. Use either dmf-dG (10-1029-xx) with standard monomers and Cap A or use UltraMILD monomers (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ) and UltraMILD Cap Mix A (40-4210-xx/ 40-4212-xx)

Deprotection: If standard monomers were used, deprotect in tert-butylamine/water 1:3 (v/v) for 6 hours at 60 °C.  If UltraMILD monomers were used, deprotect in 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_UltraMild_Deprotection.pdf). 

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 24 hours

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1057-95
50µm
250.00
10-1057-90
100µm
495.00
10-1057-02
0.25g
975.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: Dye Quencher Plot

RESPONSE:Dye Quencher Plot


DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1057-020.25grams.25grams1.9150.3330.218.8813.7310.072.52
10-1057-90100µmoles.131grams120127.55.4541
10-1057-9550µmoles.066grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1057-020.25grams.25grams2.85.0750.631.63233.16
10-1057-90100µmoles.131grams1.5.0723.614.7510.731.48
10-1057-9550µmoles.066grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

01/20/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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