Glen Research Product Information

Catalog Number: 10-1053-xx
Description: 5-OH-dU-CE Phosphoramidite

5'-Dimethoxytrityl-5-O-Acetyl-2'-deoxyUridine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite

Formula: C41H49N4O10
M.W.: 788.83 
F.W.: 306.17 

Diluent: Anhydrous Acetonitrile

Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA:10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature or 2 hours at room temperature in Ammonium Hydroxide.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1053-90
100µm
225.00
10-1053-02
0.25g
675.00

Related Document(s)

  

Literature Highlight(s)

EXTINCTION DATA

EXTINCTION DATA

ItemNucleosideλMax-1Emax-1λMax-2Emax-2E260
(nm)(ml/µmole)nm(ml/µmole)(ml/µmole)
10-10535-OH-dU2807.84.9

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1053-020.25grams.25grams3.1792.3355.434.6325.1818.474.62
10-1053-90100µmoles.079grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1053-020.25grams.25grams4.73.0788.255.1340.095.51
10-1053-90100µmoles.079grams1.5.0723.614.7510.731.48

 

 

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01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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