Glen Research Product Information

Catalog Number: 10-1040-xx
Description: dI-CE Phosphoramidite

5'-Dimethoxytrityl-2'-deoxyInosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite

Formula: C40H47N6O7
M.W.: 754.79 
F.W.: 314.19 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Note inosine is somewhat susceptible to damage by iodine during oxidation. If there are >6 incorporations of inosine within the sequence, use 0.5M CSO in anhydrous acetonitrile (40-4632-xx) and a 3 minute oxidation time for best results.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1040-90
100µm
50.00
10-1040-02
0.25g
120.00

Related Document(s)

  

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What is the base-pairing specificity of dI in an oligonucleotide?

RESPONSE:Deoxy-Inosine is often used as a degenerate base in an oligonucleotide probe or primer. This is possible since the structure allows it to base pair with all four bases in various wobble structures. However the base-pairing is not equivalent with each of the 4 naturally occuring bases. The overall preferential order of base-pairing is.

dI-dC > dI-dA > dI-dG = dI-dT

REFERENCE(S):
Martin,F.H. et.al., (1985), Nucleic Acids Res., 13, 8927-8938.
Case-Green,S. C., Southern, E.M., (1994), Nucleic Acids Res., 22, 131-136.


EXTINCTION DATA

ItemNucleosideλMax-1Emax-1λMax-2Emax-2E260
(nm)(ml/µmole)nm(ml/µmole)(ml/µmole)
10-1040dI (Inosine)24912.57.5

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1040-020.25grams.25grams3.319758.236.3826.4519.44.85
10-1040-90100µmoles.075grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1040-020.25grams.25grams4.94.0792.457.75425.78
10-1040-90100µmoles.075grams1.5.0723.614.7510.731.48

 

 

VIEW PRINTABLE VERSION

 

 


  

05/27/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2018