Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Note inosine is somewhat susceptible to damage by iodine during oxidation. If there are >6 incorporations of inosine within the sequence, use 0.5M CSO in anhydrous acetonitrile (40-4632-xx) and a 3 minute oxidation time for best results.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites
QUESTION: What is the base-pairing specificity of dI in an oligonucleotide?
RESPONSE:Deoxy-Inosine is often used as a degenerate base in an oligonucleotide probe or primer. This is possible since the structure allows it to base pair with all four bases in various wobble structures. However the base-pairing is not equivalent with each of the 4 naturally occuring bases. The overall preferential order of base-pairing is.
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.