Glen Research Product Information

Catalog Number: 10-1039-xx
Description: Amino-Modifier C6 dT

5'-Dimethoxytrityl-5-[N-(trifluoroacetylaminohexyl)-3-acrylimido]- 2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C50H62N6O10F3
M.W.: 995.05 
F.W.: 458.41 

Diluent: Anhydrous Acetonitrile

Coupling: Amino-Modifier C6 dT reacts in a manner identical to normal phosphoramidites. To prevent side reactions, synthesize using acetyl-protected dC. See Deprotection for futher details.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. The TFA protecting group is removed during standard ammonium hydroxide deprotection. A minor side reaction during ammonia deprotection can lead to irreversibly capping 2-5% of the amine. This could be significant if multiple additions of the modifier are made. To prevent the reaction, synthesize using acetyl-protected dC and deprotect in 30% ammonia/40% methylamine 1:1 (AMA) at 65°C for 15 minutes.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1039-90
100µm
180.00
10-1039-02
0.25g
360.00
10-1039-05
0.5g
720.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: What is the extinction coefficient of Amino-Modifier C6 dT and C2 dT?

RESPONSE:The extinction coefficient of DNA at 260nm is around 10,000/mole or 10/µmole. The extinction coefficient of Amino-Modifier C6 dT and C2 dT is around 8.7. The synthesis of these monomers does not pass through the deoxynucleoside and this figure is estimated by comparison to dT-CE Phosphoramidite.

Note: dA=15.4, dC=7.4, dG=11.5, T=8.7


DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1039-020.25grams.25grams2.5170.3342.226.3819.1814.073.52
10-1039-050.5grams.5grams5.0215492.457.754230.87.7
10-1039-90100µmoles.1grams120127.55.4541
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1039-020.25grams.25grams3.75.0768.642.8831.184.29
10-1039-050.5grams.5grams7.5.07143.689.7565.278.98
10-1039-90100µmoles.1grams1.5.0723.614.7510.731.48

 

 

VIEW PRINTABLE VERSION

 

 


  

12/12/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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