Glen Research Product Information

Catalog Number: 10-1034-xx
Description: 4-Thio-dT-CE Phosphoramidite

5'-Dimethoxytrityl-2'-deoxy-4-(2-cyanoethylthio)-Thymidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C43H52N5O7PS 
M.W.: 813.95 
F.W.: 320.26 

Diluent: Anhydrous Acetonitrile

Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation.

Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 2hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1034-95
50µm
165.00
10-1034-90
100µm
295.00
10-1034-02
0.25g
675.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: Can 4-thiodU be used for crosslinking and how is it prepared?

RESPONSE:4-thioU is efficiently activated for crosslinking by exposure to long-wavelength UV light for up to 10 minutes. Crosslinks are formed with RNA and proteins.

Oligonucleotides containing 4-thiodU (10-1051) and 4-thioT (10-1033) can be produced from the triazole modified phosphoramidites using the convertible nucleoside strategy (1,2). However, we now offer the nuclesides 4-thio-dT (10-1034), 4-thio-dU (10-1052), 2-thio-dT (10-1036), and 4-thio-U (10-3052) for direct incorporation into oligonucleotides and the convertible monomers have been discontinued. A further enhancement of this strategy used 4-thioxU as an intermediated in the preparation of thiocarbonyl crosslinkers (3).

REFERENCE(S):
(1) Y.Z. Xu, Q. Zheng, and P.F. Swann, J. Org. Chem., 1992, 57, 3841.
(2) The Glen Report, 1993, 6.1, 1, and references cited therein.
(3) R.S. Coleman and J.M. Siedlecki, Journal of the American Chemical Society, 1992, 114, 9229-9230.


DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1034-020.25grams.25grams3.078953.433.3824.2717.84.45
10-1034-90100µmoles.081grams120127.55.4541
10-1034-9550µmoles.041grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1034-020.25grams.25grams4.58.0785.253.2538.735.33
10-1034-90100µmoles.081grams1.5.0723.614.7510.731.48
10-1034-9550µmoles.041grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

12/12/2018

 

 

 

 

 

 

 

 

 

 

 

 

 

 


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2018