Glen Research Product Information

Catalog Number: 10-1028-xx
Description: 8-oxo-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite

Formula: C44H54N7O9
M.W.: 855.93 
F.W.: 345.21 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 24 hours

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1028-95
50µm
177.50
10-1028-90
100µm
355.00
10-1028-02
0.25g
975.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: I have 8-oxo-dG in an oligo with a dye that is not compatible with deprotection in ammonium hydroxide. Can I deprotect an oligo containing 8-oxo-dG in AMA?

RESPONSE:Yes. 8-oxo-dG can be cleanly deprotected in AMA with 1 hour at 55 °C.


EXTINCTION DATA

ItemNucleosideλMax-1Emax-1λMax-2Emax-2E260
(nm)(ml/µmole)nm(ml/µmole)(ml/µmole)
10-10288-oxo-dG2945.22506.75.9

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1028-020.25grams.25grams2.928450.431.522.9116.84.2
10-1028-90100µmoles.086grams120127.55.4541
10-1028-9550µmoles.043grams.53.3321.25.91.67.17
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1028-020.25grams.25grams4.36.0780.850.536.735.05
10-1028-90100µmoles.086grams1.5.0723.614.7510.731.48
10-1028-9550µmoles.043grams.75.078.65.383.91.54

 

 

VIEW PRINTABLE VERSION

 

 


  

01/16/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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