Glen Research Product Information

Catalog Number: 10-1015-xx
Description: Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite

Formula: C41H50N5O8
M.W.: 771.85 
F.W.: 289.18 

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Storage: Controlled room temperature or lower, dry

Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

   

Pricing Information

Catalog Number

Pack Size

Price (US$)


10-1015-02
0.25g
12.50
10-1015-05
0.5g
25.00
10-1015-10
1.0g
50.00
10-1015-20
2.0g
100.00
10-1015-40
4.0g
200.00
10-1015-C2
0.25g
12.50
10-1015-C5
0.5g
25.00
10-1015-1C
1.0g
50.00
10-1015-2C
2.0g
100.00
10-1015-5HT
5g
250.00
10-1015-02M
0.25g
12.50
10-1015-05M
0.5g
25.00
10-1015-10M
1.0g
50.00
10-1015-5S
5.0g
250.00
10-1015-1S
10.0g
500.00
10-1015-50
5.0g
250.00

Related Document(s)

  

Literature Highlight(s)

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: How does this work? What would happen if I used the AMA reagent with Bz protected dC?

RESPONSE:dC when protected as an amide linkage is susceptible to transamination or displacement of the amide with the deprotecting amine. Normally, the deprotecting amine is ammonia and displacement leads to the same product as hydrolysis. However, if methylamine is used for the deprotection step with a benzoyl protected dC, approximately 10% of the product is from the displacement reaction to give N-methyl-dC residues, while the remaining 90% is the desired product formed by hydrolysis. A more labile protecting group on dC is isobutyryl which is also commercially available. This group is displaced by methylamine to about 5% - an improvement but still a mutation. Ac-dC, on the other hand, is hydrolyzed virtually instantaneously by the AMA reagent and none of the slower displacement reaction gets a chance to occur.

REFERENCE(S):
M.P. Reddy, Beckman Instruments, personal communication.


QUESTION: Can the AMA reagent be made up and stored? for how long? in what kind of container?

RESPONSE:Yes. Store tightly sealed in a glass bottle in the refrigerator. The reagent does not decompose but routine opening will lose some of the volatile bases. Dispose properly after 4-6 weeks.

REFERENCE(S):
Candy Lee, Dartmouth


DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-1015-020.25grams.25grams3.2494.6756.835.525.8218.934.73
10-1015-050.5grams.5grams6.48202.67121.67655.2740.5310.13
10-1015-101.0gram1grams12.96418.67251.2157114.1883.7320.93
10-1015-202.0grams2grams25.91850.33510.2318.88231.91170.0742.52
10-1015-404.0grams4grams51.8217141028.4642.75467.45342.885.7
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-1015-1C1.0gram1grams19.34.07383.6239.75174.3623.98
10-1015-2C2.0grams2grams38.67.07770.2481.38350.0948.14
10-1015-C20.25grams.25grams4.83.0793.458.3842.455.84
10-1015-C50.5grams.5grams9.67.07190.2118.8886.4511.89

 

 

VIEW PRINTABLE VERSION

 

 


  

01/19/2019

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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