INSTRUMENT REAGENT USAGE

ABI Instruments

The older ABI 392 and 394 instruments use a 0.1M concentration for monomers. The monomers are diluted with anhydrous acetonitrile as follows:

Pack

Volume

50µmoles

0.5mL (500µL)

100µmoles

1.0mL (1000µL)

0.25g and greater

See analytical data sheet,Product Information web page, Or Physical Data at the rear of the catalog.

These instruments use the following volumes per cycle:

Cycle

Volume (µL)

40nmole

80

0.2µmole

110

1µmole

150

10µmole

600

LV40

30

LV200

50

Prime (2 seconds)*

400

*An abbreviated prime procedure should be used for precious minor bases and modifiers.

 

Expedite Instruments

For Expedite instruments, monomers are diluted at a rate of 0.5g per 10mL which is a 0.067M concentration for dT (0.058M for dA). The monomers are diluted to 0.067Mwith anhydrous acetonitrile as follows:

Pack

Volume

50µmoles

0.75mL (750µL)

100µmoles

1.5mL (1500µL)

0.25g and greater

1.5X dilution for 0.1M

See analytical data sheet,roduct Information web page,Or Physical Data at the rear of the catalog.

These instruments use the following volumes per cycle:

Cycle

Volume (µL)

50nmole

50

0.2µmole

80

1µmole

112

15µmole

800

Prime (amidites)*

160

Prime (individual)*

320

*An abbreviated prime procedure should be used for precious minor bases and modifiers.

 

Beckman Instruments

For Beckman instruments, monomers are diluted at a rate of 0.5g per 10mL which is a 0.067M concentration for dT (0.058M for dA). The monomers are diluted to 0.067Mwith anhydrous acetonitrile as follows:

Pack

Volume

50µmoles

0.75mL (750µL)

100µmoles

1.5mL (1500µL)

0.25g and greater

1.5X dilution for 0.1M

See analytical data sheet,roduct Information web page,Or Physical Data at the rear of the catalog.

These instruments use the following volumes per cycle:

Cycle

Volume (µL)

30nmole

50

200nmole

100

1000nmole

200

Prime

240

 

MerMade Synthesizer

For MerMade synthesizers, monomers are diluted at a rate of 5g per 100mL and 10g per 200mL. This is a 0.067M concentration for dT (0.058M for dA).

Note: These figures are for estimation purposes only. Contact the instrument manufacturer for exact numbers.

Prime volumes shown are for regular monomers. An abbreviated prime procedure should be used for precious minor bases and modifiers. Check with the instrument manufacturer for details. We offer our procedures for ABI and Expedite Instruments.

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