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INSTRUMENT REAGENT USAGE
ABI Instruments
The older ABI 392 and 394 instruments use a 0.1M concentration for monomers. The monomers are diluted with anhydrous acetonitrile as follows:
|
Pack |
Volume |
|
50µmoles |
0.5mL (500µL) |
|
100µmoles |
1.0mL (1000µL) |
|
0.25g and greater |
See analytical data sheet,Product Information web page, Or Physical Data at the rear of the catalog. |
These instruments use the following volumes per cycle:
|
Cycle |
Volume (µL) |
|
40nmole |
80 |
|
0.2µmole |
110 |
|
1µmole |
150 |
|
10µmole |
600 |
|
LV40 |
30 |
|
LV200 |
50 |
|
Prime (2 seconds)* |
400 |
Expedite Instruments
For Expedite instruments, monomers are diluted at a rate of 0.5g per 10mL which is a 0.067M concentration for dT (0.058M for dA). The monomers are diluted to 0.067Mwith anhydrous acetonitrile as follows:
|
Pack |
Volume |
|
50µmoles |
0.75mL (750µL) |
|
100µmoles |
1.5mL (1500µL) |
|
0.25g and greater |
1.5X dilution for 0.1M See analytical data sheet,roduct Information web page,Or Physical Data at the rear of the catalog. |
These instruments use the following volumes per cycle:
|
Cycle |
Volume (µL) |
|
50nmole |
50 |
|
0.2µmole |
80 |
|
1µmole |
112 |
|
15µmole |
800 |
|
Prime (amidites)* |
160 |
|
Prime (individual)* |
320 |
Beckman Instruments
For Beckman instruments, monomers are diluted at a rate of 0.5g per 10mL which is a 0.067M concentration for dT (0.058M for dA). The monomers are diluted to 0.067Mwith anhydrous acetonitrile as follows:
|
Pack |
Volume |
|
50µmoles |
0.75mL (750µL) |
|
100µmoles |
1.5mL (1500µL) |
|
0.25g and greater |
1.5X dilution for 0.1M See analytical data sheet,roduct Information web page,Or Physical Data at the rear of the catalog. |
These instruments use the following volumes per cycle:
|
Cycle |
Volume (µL) |
|
30nmole |
50 |
|
200nmole |
100 |
|
1000nmole |
200 |
|
Prime |
240 |
MerMade Synthesizer
For MerMade synthesizers, monomers are diluted at a rate of 5g per 100mL and 10g per 200mL. This is a 0.067M concentration for dT (0.058M for dA).
Note: These figures are for estimation purposes only. Contact the instrument manufacturer for exact numbers.
Prime volumes shown are for regular monomers. An abbreviated prime procedure should be used for precious minor bases and modifiers. Check with the instrument manufacturer for details. We offer our procedures for ABI and Expedite Instruments.