-GLen research Corporation-
FOUNDED TO PROVIDE REAGENTS AND SERVICES FOR DNA RESEARCH BASED ON
TECHNICAL EXCELLENCE AND WITH QUALITY ASSURED.
The Glen Research concept was based on our perception
that the research community required a supply of novel reagents which,
at first glance, may have seemed of little commercial value to the larger
suppliers. We saw these esoteric products as the potential vehicles
for the development of novel strategies for use in diagnostics, sequencing,
and therapeutics. At the same time, we foresaw a need to provide products
for routine oligonucleotide synthesis backed with quality specifications,
analytical techniques and certification at the highest level.
The initial Glen Research offering in 1987 contained
a complete set of reagents for DNA synthesis for cyanoethyl (CE) phosphoramidite
chemistry which then was rapidly gaining acceptance. Modest though this
range may have been, it was of major significance due to the Certificates
of Analysis which accompanied every product. The analytical techniques
and quality criteria used for the evaluation and acceptance of these
reagents were to become an industry standard many years later.
This rather routine beginning was soon followed by
the products which have become the Glen Research specialty: the largest
selection of minor base phosphoramidites available and a potentially
bewildering variety of reagents for oligonucleotide modification. To
aid researchers in deciphering this array of products and to prompt
them as to potential uses, the Glen Research Report was launched. Now
published semi-annually as The Glen Report, this unique, valuable and
respected newsletter has introduced an astonishing array of novel reagents
while elucidating some complex chemical strategies and techniques. Today,
Glen Research still offers far and away the widest selection of minor
base phosphoramidites, modifiers and reagents for labelling oligonucleotides
during the synthesis.
In 1989, Glen Research expanded into the delicate art
of RNA synthesis, introducing the set of monomers still respected as
the best available for the vast majority of applications. And, in 1990,
we launched what was to become the most comprehensive range of products
for the synthesis of the nuclease resistant variant 2'-OMe-RNA.
During 1993, Glen Research announced the Sterling line
of products for DNA synthesis based on the highest criteria of quality,
purity and reproducibility. Sterling grade is the level to which all
other suppliers must aspire and which will lead the way to the development
of oligonucleotide therapeutics.
As the market for oligonucleotides, modified and unmodified,
continues to grow, Glen Research is committed to continuing to introduce
novel reagents and techniques while maintaining our existing product
mix and high level of customer service.
DEDICATED TO THE DEVELOPMENT AND SUPPLY OF NOVEL PRODUCTS AND TECHNIQUES
FOR USE IN DNA AND RNA RESEARCH.
The list of minor base phosphoramidites offered by
Glen Research is unique and comprehensive in its range. The uses applied
by researchers to these monomers are extremely far-reaching, extending
from structural analysis using X-ray crystallography and spectroscopic
techniques, through mutagenesis studies and endonuclease assays, to
triplex and antisense research. Of particular note is the commercial
introduction of 5-propynyl-pyrimidine nucleoside phosphoramidites, under
license from Gilead Sciences, Inc., which show tremendous potential
in the development of antisense therapeutics. The minor base subset
of our product line is a tribute to the ingenuity of our customers and
we will continue to expand it to meet their needs.
With the launch in 1987 of a complete line of Amino-
and Thiol-Modifiers, Glen Research has contributed to the development
of a multitude of research techniques, including diagnostic probes after
labelling with haptens or enzymes, attachment of oligos to solid supports
for affinity chromatography or dot blot assays, and DNA sequencing.
More recently, synthesis of labelled oligonucleotides has been greatly
simplified by the emergence of phosphoramidites of the haptens themselves.
For example, Glen Research currently produces biotin, fluorescein, psoralen,
cholesterol, and DNP phosphoramidites. Glen Research supports this line
of products with a comprehensive and well-referenced technical booklet
User Guide to DNA Modification and Labelling.
Glen Research introduced its line of RNA monomers after
a detailed review of the various protecting schemes available. Since
their launch, these monomers have been applied successfully in a variety
of RNA research environments. Our customers have reported excellent
results in the synthesis of RNA used for: structural studies by spectroscopic
analysis; analysis of ribozyme function; studies on structure and activity
relationships in RNA viruses; hybridization studies; and ribonuclease
assays. We acknowledge that even with these high-quality monomers, oligoribonucleotides
are still difficult and time-consuming to produce and further work is
continuing to improve synthetic procedures.
At an early stage, Glen Research noted the potential
advantages of naturally occurring and nuclease resistant 2'-OMe-RNA.
Although monomers for this chemistry proved to be difficult to produce,
we now offer the widest variety of 2'-OMe-RNA monomers for use in antisense
therapeutic and probe experiments, ribozyme studies, and other physical
studies. In comparison to normal RNA, the ease of synthesis of 2'-OMe-RNA
is remarkably improved with purification and isolation techniques identical
to those used for DNA.
Modification of the phosphate backbone is an area of
interest in the development of oligonucleotide therapeutics. Glen Research
supports a variety of simple synthetic schemes. These include preparation
of phosphorothioates during routine synthesis using a sulfurization
step in place of the routine oxidation step, use of H-phosphonate monomers
to prepare phosphoramidate or phosphotriester linkages, and the production
of methyl phosphonate linkages using methyl phosphonamidite monomers.
PRODUCTS CERTIFIED TO BE THE HIGHEST PURITY AND PERFORMANCE AVAILABLE
FOR DNA SYNTHESIS.
Reagent quality in solid-phase synthesis is of paramount
importance because of the excess used every cycle. Even trace impurities
can exert a significant influence in the quality of the final product.
Glen Research has always supplied detailed Certificates of Analysis
to substantiate reagent quality, although providing this information
is still resisted by many of the current reagent suppliers. Glen Research
quality parameters, based on our understanding of the needs of solid-phase
synthesis, remain the tightest in the industry. Since Glen Research
reagents for the major DNA synthesizers are manufactured with such
a high degree of production control and are analyzed to such tight
specifications, they have been accepted as the ultimate in performance
Thus, in 1993, Glen Research accredited this important
concept in a separate brochure introducing our Sterling grade. Every
item in this line is produced using the highest quality materials
available under the most stringent and reproducible conditions of
manufacture. These criteria are supported with Sterling Certificates
of Analysis that describe the rigorous analytical techniques and provide
the actual analytical results. Sterling products are available for
all major DNA synthesizers and include CE phosphoramidite monomers,
synthesis columns, and all liquid reagents.
An ingenious modification of the protecting groups
used in DNA synthesis offers a potentially significant improvement
to existing techniques. By replacing benzoyl with acetyl as the protecting
group for dC, synthesized oligos can be cleaved and deprotected in
10 minutes. This UltraFast deprotection scheme, originally developed
by Beckman Instruments, Inc., promises to accelerate the production
or PCR and sequencing primers without the need to change to novel
and less well tried chemistry.
As we endeavor to improve on existing products for
DNA synthesis, we focus on areas of weakness in the synthesis process.
Our work on the redesign of synthesis columns has culminated in the
development of TWIST columns for routine synthesis. In addition
to being virtually leak-proof, TWIST columns allow users, if necessary,
to gain access to the support simply by twisting off the retaining
Purification and isolation of synthetic oligodeoxynucleotides
remains the most time-consuming and labor-intensive portion of the
synthesis process. We are happy to have participated in alleviating
this production bottleneck with the introduction of the Poly-Pak
group of DNA purification products. Poly-Pak cartridges allow users
to purify and isolate synthetic oligos in a fraction of the time needed
in the past. This technique has gained popularity with core facilities,
commercial producers, as well as with individual researchers. Glen
Research supports the technique with a comprehensive User Guide to
TWIST and Poly-Pak
are trademarks of Glen Research Corporation