Diluent: Anhydrous ACN, DMSO or DMF |
| Coupling: For labeling 0.2 umoles of an amino-modified oligonucleotide, dissolve 2.3 mg of Azidobutyrate NHS Ester in 60 uL of ACN (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500 uL of 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12 uL of the Azidobutyrate NHS Ester solution to the dissolved oligonucleotide and let react for 2 hours at room temperature and desalt. |
| Deprotection: Not Applicable |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
