home


CATALOG PRODUCTS ARCHIVES FEEDBACK SEARCH
  alphbetical index Catalog Number index MSDS index Deprotection Volumes 1-5 User guide to Purification Literature Highlights Listing of oligo houses FAQ  

Catalog Number: 40-4037-xx

Description: Sulfurizing Reagent II

3-((N,N-dimethylaminomethylidene)amino)-3H-1,2,4-dithiazole-5-thione
Formula: C5H7N3S3M.W.: 205.31

Diluent: 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
*For a 0.05M solution:
Fully dissolve 1g in 60mL pyridine, and dilute with 40mL acetonitrile,
Fully dissolve 2g in 120mL pyridine, and dilute with 80mL acetonitrile
See technical bulletin for details.
Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
Deprotection: See TB_Sulfurizing II.pdf Technical Bulletin
Storage: Controlled room temperature or lower, dry
Stability in Solution: At least 6 months
Catalog Information: Backbone Modification
Material Safety Data Sheet

Literature Highlights

Glen Report 18.1: Sulfurizing Reagent Ii - Stable In Solution And Optimized For Rna Sulfurization
Glen Report 18.1 Supplement: Sulfurizing Reagent Ii And Its Use In Synthesizing Oligonucleotide Phosphorothioates

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.Pack
Size
Grams/
Pack
.05M Dil.
(mL)
LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
40-4037-101gram1gramsN/AN/AN/AN/AN/AN/AN/A
40-4037-202grams2gramsN/AN/AN/AN/AN/AN/AN/A
Expedite
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
40-4037-101gram1gramsN/AN/AN/AN/AN/A
40-4037-202grams2gramsN/AN/AN/AN/AN/A
Mermade
Cat.No.Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity30nm200nm1000nm
Approximate Number of Additions
40-4037-101gram1gramsN/AN/AN/AN/A
40-4037-202grams2gramsN/AN/AN/AN/A

12/15/2014 | http://www.glenres.com/ProductFiles/40-4037.html