Catalog Number: 20-5932-xx Description: 3'-BHQ-2 CPG4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-ethyl-
2-O-(4,4'-dimethoxytrityl))-N-ethyl-2-O-glycolate-CPG | | | F.W.: 556.47 |
Diluent: Anhydrous Acetonitrile | | Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. | | Deprotection: Cleavage of the oligonucleotide from this support requires a minimum of 20 minutes at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. | | Storage: Freezer storage, -10 to -30°C, dry Light sensitive | | Stability in Solution: 2-3 days | | Please Note: 'Black Hole Quencher', 'BHQ-0', 'BHQ-1', 'BHQ-2' and 'BHQ-3' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged. |
Literature Highlights
Frequently Asked Technical QuestionQUESTION: What are the physical properties of the various black hole quenchers? RESPONSE: | Quencher |
lmax (nm) | E260 (L/mol.cm) | Emax (L/mol.cm) | | BHQ-0
| 493 | 7,700 | 34,000 | | BHQ-1 | 534 | 8,000 | 34,000 | | BHQ-2
| 579 | 8,000 | 38,000 | | BHQ-3 | 672 | 13,000
| 42,700 |
REFERENCE(S):
Glen Report 17.1
11/14/2008 | http://www.glenres.com/ProductFiles/20-5932.html |
