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Catalog Number: 20-5910-xx
Description: 3'-TAMRA CPG
| 1-Dimethoxytrityloxy-3-[O-(N-carboxy-(Tetramethyl-rhodamine)- 3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG | ||
| F.W.: 623.60 | ||
Diluent: Not Applicable |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Technical Bulletin for details (www.glenresearch.com/Technical/TB_UltraMild_Deprotection.pdf).Note: A side reaction can occur that leads to a loss of the TAMRA label. Treatment of the support prior to cleavage/deprotection with a hindered base, such as 10% diethylamine in ACN, for 3-5 minutes will minimize the loss of label. |
| Storage: Freezer storage, -10 to -30°C, dry. Light sensitive material. |
| Stability in Solution: Not Applicable |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 10.1: NEW FLUORESCENT REAGENTS - TAMRA CPG, FLUORESCEIN-dT |
| Glen Report 12.1: POLY-PAK PURIFICATION OF LABELLED PROBES |
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html
REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html
QUESTION: What are the relative extinction coefficients of various dyes?
RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes
QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?
RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.
11/04/2011 | http://www.glenres.com/ProductFiles/20-5910.html