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Catalog Number: 20-2961-xx
Description: 3'-(6-FAM) CPG
| 1-Dimethoxytrityloxy-3-[O-(N-carboxy-(di-O-pivaloyl-fluorescein)- 3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG | ||
| F.W.: 569.46 | ||
Diluent: Not Applicable |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: Not Applicable |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 11.2: MORE NOVEL MONOMERS |
| Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html
REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html
QUESTION: What are the relative extinction coefficients of various dyes?
RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes
QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?
RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.
11/04/2011 | http://www.glenres.com/ProductFiles/20-2961.html