 |
 |
|||||||||
 |
 |
 |
 |
 |
 |
 |
 |
 |
||
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
|
||||||||||||||||||||||||||
Catalog Number: 20-2013-xx
(20-2113-xx, 20-2213-xx)
Description: Ac-dC-CPG 500
| 5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine, 3'-succinoyl-long chain alkylamino-CPG 500 | ||
| F.W.: 289.18 | ||
Diluent: Not Applicable |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Deprotect using the protocol required by the nucleobases. |
| Storage: Controlled room temperature or lower, dry |
| Stability in Solution: Not Applicable |
| Catalog Information | Material Safety Data Sheet |
Frequently Asked Technical Question
QUESTION: What is the procedure for deprotecting with AMA?
RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium
hydroxide and aqueous MethylAmine. With AMA the cleavage of the
oligonucleotide from the support is accomplished in 5 minutes at room
temperature. The deprotection step is carried out at 65°C for a
further 5 minutes. Deprotection can also be carried out at lower
temperatures as shown in Table 1. In all cases, no base modification
has been observed.
TABLE 1: DEPROTECTION WITH AMA
| Time | Temperature |
| 5 min | 65°C |
| 10 min | 55°C |
| 30 min | 37°C |
| 90 min | 25°C |
08/15/2012 | http://www.glenres.com/ProductFiles/20-2013.html